Determination of a Serum 25-Hydroxyvitamin D Reference Ranges in Japanese Adults Using Fully Automated Liquid Chromatography-Tandem Mass Spectrometry.

Miyamoto, H ; Kawakami, D ; et al.

In: The Journal of nutrition, Jg. 153 (2023-04-01), Heft 4, S. 1253

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Background: Despite an increasing interest in vitamin D status, a reference range of the nutrient has not been fully established. This is partly due to a paucity of standardized measuring systems with high throughput. In addition, the range may vary by populations and may change with modernization of lifestyles.

Objectives: This study aims to calculate the current reference concentration of 25-hydroxyvitamin D (25(OH)D) among healthy people living in an urban area in Japan.

Methods: A newly developed fully automated liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) system was used to measure serum 25(OH)D concentrations. Reproducibility was assessed by measuring standardized samples. Accuracy was validated by comparing with commercially available immunoassays. Then, mass screening was conducted targeting participants who received medical checkups in Tokyo from April 2019 to March 2020, and the reference ranges were calculated.

Results: The coefficients of variations of interoperator and interday reproducibility were 4.1%-8.5% and 3.7%-8.0% for 25-hydroxyvitamin D 2 (25(OH)D2) and 4.7%-7.0% and 4.0%-6.9% for 25-hydroxyvitamine D3, respectively. The measured total 25(OH)D concentrations correlated well with those measured by immunoassays. In total, 5518 participants were measured for 25(OH)D concentrations, among whom 98% showed inadequate concentrations (<30 ng/mL). The reference ranges of total 25(OH)D for female, male, and total participants were 7-30 ng/mL, 5-27 ng/mL, and 6-29 ng/mL, respectively. After excluding those with abnormal renal and liver function, the range was 6-30 ng/mL.

Conclusions: The high prevalence of vitamin D insufficiency among seemingly healthy population may be attributed to lifestyle characteristics of people living in urban areas of Japan, including spending less time outdoors and lower intake of traditional foods. Longitudinal follow-up and mass screenings targeting different population will help elucidate reasons for discrepancies between official guidelines and the observed concentrations, to which the well-validated measurement system is essential.

Titel:	Determination of a Serum 25-Hydroxyvitamin D Reference Ranges in Japanese Adults Using Fully Automated Liquid Chromatography-Tandem Mass Spectrometry.
Autor/in / Beteiligte Person:	Miyamoto, H ; Kawakami, D ; Hanafusa, N ; Nakanishi, T ; Miyasaka, M ; Furutani, Y ; Ikeda, Y ; Ito, K ; Kato, T ; Yokoyama, K ; Arakawa, S ; Saito, M ; Furusho, T ; Matsuura, T ; Ochi, S
Link:	Full Text Finder (Volltext)
Zeitschrift:	The Journal of nutrition, Jg. 153 (2023-04-01), Heft 4, S. 1253
Veröffentlichung:	2023- : [New York, NY] : Elsevier ; <i>Original Publication</i>: 1928-1933 : Springfield, Ill. : C. C. Thomas, 2023
Medientyp:	academicJournal
ISSN:	1541-6100 (electronic)
DOI:	10.1016/j.tjnut.2023.01.036
Schlagwort:	Adult Female Humans Male 25-Hydroxyvitamin D 2 Calcifediol Reference Values Reproducibility of Results Vitamins Japan epidemiology Chromatography, Liquid methods East Asian People statistics & numerical data Tandem Mass Spectrometry methods Vitamin D blood Vitamin D Deficiency blood Vitamin D Deficiency diagnosis Vitamin D Deficiency epidemiology Vitamin D Deficiency etiology
Sonstiges:	Nachgewiesen in: MEDLINE Sprachen: English Publication Type: Journal Article; Research Support, Non-U.S. Gov't Language: English [J Nutr] 2023 Apr; Vol. 153 (4), pp. 1253-1264. <i>Date of Electronic Publication: </i>2023 Feb 03. MeSH Terms: Chromatography, Liquid* / methods ; East Asian People* / statistics & numerical data ; Tandem Mass Spectrometry* / methods ; Vitamin D* / blood ; Vitamin D Deficiency* / blood ; Vitamin D Deficiency* / diagnosis ; Vitamin D Deficiency* / epidemiology ; Vitamin D Deficiency* / etiology ; Adult ; Female ; Humans ; Male ; 25-Hydroxyvitamin D 2 ; Calcifediol ; Reference Values ; Reproducibility of Results ; Vitamins ; Japan / epidemiology Contributed Indexing: Keywords: LC-MS/MS; laboratory medicine; mass screening; reference range; vitamin D Substance Nomenclature: A288AR3C9H (25-hydroxyvitamin D) ; 21343-40-8 (25-Hydroxyvitamin D 2) ; P6YZ13C99Q (Calcifediol) ; 1406-16-2 (Vitamin D) ; 0 (Vitamins) Entry Date(s): Date Created: 20230222 Date Completed: 20230419 Latest Revision: 20230611 Update Code: 20240513

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