High-quality faba bean reference transcripts generated using PacBio and Illumina RNA-seq data.
In: Scientific data, Jg. 11 (2024-04-09), Heft 1, S. 359
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Zugriff:
The genome of faba bean was first published in 2023. To promote future molecular breeding studies, we improved the quality of the faba genome based on high-density genetic maps and the Illumina and Pacbio RNA-seq datasets. Two high-density genetic maps were used to conduct the scaffold ordering and orientation of faba bean, culminating in an increased length (i.e., 14.28 Mbp) of chromosomes and a decrease in the number of scaffolds by 45. In gene model mining and optimisation, the PacBio and Illumina RNA-seq datasets from 37 samples allowed for the identification and correction 121,606 transcripts, and the data facilitated a prediction of 15,640 alternative splicing events, 2,148 lncRNAs, and 1,752 fusion transcripts, thus allowing for a clearer understanding of the gene structures underlying the faba genome. Moreover, a total of 38,850 new genes including 56,188 transcripts were identified compared with the reference genome. Finally, the genetic data of the reference genome was integrated and a comprehensive and complete faba bean transcriptome sequence of 103,267 transcripts derived from 54,753 uni-genes was formed.
(© 2024. The Author(s).)
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High-quality faba bean reference transcripts generated using PacBio and Illumina RNA-seq data.
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Autor/in / Beteiligte Person: | Zhao, N ; Zhou, E ; Miao, Y ; Xue, D ; Wang, Y ; Wang, K ; Gu, C ; Yao, M ; Zhou, Y ; Li, B ; Wang, X ; Wei, L |
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Zeitschrift: | Scientific data, Jg. 11 (2024-04-09), Heft 1, S. 359 |
Veröffentlichung: | London : Nature Publishing Group, 2014-, 2024 |
Medientyp: | academicJournal |
ISSN: | 2052-4463 (electronic) |
DOI: | 10.1038/s41597-024-03204-4 |
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