Exploring human splenic red pulp vasculature in virtual reality. Details of sheathed capillaries and the open capillary network. Supplementary material

Supplementary files demonstrate our data in an interactive application. Please download und unpack the file, then launch file "start.bat". Please note that loading the original images takes some time. If you have a Steam VR headset, it is detected and used automatically. Supplementary file 1 Localization of arteries and arterioles in ROI 4. ROI 4 with all endothelia and some periarterial fibroblasts colored blue (CD34), smooth muscle alpha-actin (SMA) colored white and capillary sheaths depicted in green (CD271). Most arteries and arterioles can be localized by white color. White appears in a vertex of the blue reconstruction of CD34 staining, if a surface reconstructed for SMA labelling is closer than 8.75 µm to this vertex. Capillary sheaths connected to the selected arterioles (marked red in Suppl. file 2) are green and non-connected sheaths fed by other arterioles are dark green (CD271). Supplementary file 2 Relation of capillary sheaths to terminal arterioles in ROI4. ROI 4 with all endothelia and some periarterial fibroblasts colored blue (CD34), selected terminal artery and terminal arterioles manually labelled red and capillary sheaths connected to the labelled arterioles in green (CD271). Non-connected sheaths fed by other arterioles are dark green (CD271). Follicular dendritic cells (FDCs) in follicles are light green (CD271). This file corresponds to Fig. 3 and to Suppl. Video 1-3. Supplementary file 3 Relation of capillary sheaths to terminal arterioles in ROI 2. ROI 2 with all endothelia and some periarterial fibroblasts colored blue (CD34), selected terminal artery and terminal arterioles manually labelled red and capillary sheaths connected to the labelled arterioles in green (CD271). Non-connected sheaths fed by other arterioles are dark green (CD271). FDCs in follicles are light green (CD271). This file corresponds to Fig. 4a,b. Supplementary file 4 Relation of capillary sheaths to terminal arterioles in ROI 1. ROI 1 with all endothelia and some periarterial fibroblasts colored blue (CD34), ...